locus

BSU13880

BSU_13880

proteinLength

285

288

geneLength

855

867

outlinks

bsu

BSU13880

BSU_13880

The protein

Protein family

[SW|PRD-containing transcription factors|transcription antiterminator] of the BglG/ [[protein|SacY]] family

[SW|PRD-containing transcription factors]

The protein

Paralogous protein(s)

[[protein|LicT]], [[protein|SacT]], [[protein|SacY]]

[[this]]

The protein

Structure

[PDB|3GWH] ([SW|PRD]-II) [Pubmed|19684596]

[PDB|3RIO] (RBD-[SW|PRD]-I) [Pubmed|22750856]

[PDB|3GWH] ([SW|PRD]-II) [Pubmed|19684596]

Biological materials

Mutant

available in [SW|Jörg Stülke]'s lab:

GP109 (in frame deletion)

GP926 (Δ[[gene|glcT]]-[[gene|ptsG]]::tet) [Pubmed|22722928]

BKE13880 (Δ[[gene|glcT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATACCTCATATCGT, downstream forward: _UP4_TAAATTCAGTTTATCCTTAT

BKK13880 (Δ[[gene|glcT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATACCTCATATCGT, downstream forward: _UP4_TAAATTCAGTTTATCCTTAT

available in [SW|Jörg Stülke]'s lab:

GP109 (Δ[[gene|glcT]], in frame deletion)

GP926 (Δ[[gene|glcT]]-[[gene|ptsG]]::tet) [Pubmed|22722928]

BKE13880 (Δ[[gene|glcT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATACCTCATATCGT, downstream forward: _UP4_TAAATTCAGTTTATCCTTAT

BKK13880 (Δ[[gene|glcT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATACCTCATATCGT, downstream forward: _UP4_TAAATTCAGTTTATCCTTAT

Biological materials

Expression vector

pGP124 (full length, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP114 (amino acids 1-60, RNA-binding domain, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP164 (both PRDs, in [SW|pWH844]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP424 (PRDI, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP425 (PRDII, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP442 (PRDI, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab

pGP443 (PRDII, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab

pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Jörg Stülke], University of Göttingen, Germany

[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

Original publications

11902727, 9765562, 12437213, 10543968, 17074746, 15155854, 14527945, 19684596, 22722928, 20939030, 22750856

11902727, 9765562, 12437213, 10543968, 17074746, 15155854, 14527945, 19684596, 22722928, 20939030, 22750856, 30082753

Biological materials

Expression vectors

pGP124 (full length, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP114 (amino acids 1-60, RNA-binding domain, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP164 (both PRDs, in [SW|pWH844]), in addition diverse Expression vector for phosphorylation site mutants and for RBD mutants (all in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP424 (PRDI, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP425 (PRDII, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP442 (PRDI, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab

pGP443 (PRDII, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab

pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]